Vectors with the E.coli BirA biotin ligase

BirA in pENTR1A (w860-1)

High resolution pdf: BirA in pENTR1A (w860-1).pdf

Sequence file: BirA in pENTR1A (w860-1).seq

Sequence verification files: w860-1ENTRforw.ab1, w860-1ENTRrev.ab1

Addgene #: 29645

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin (50 ug/ml final concentration).

Compatible Destination vectors: pLenti CMV, CMV/TO, CMVtight, CMVTRE3G, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO.

 

pLenti PGK BirA Hygro (w874-1)

High resolution pdf: pLenti PGK BirA Hygro (w874-1).pdf

Sequence file: pLenti PGK BirA Hygro (w874-1).seq

Addgene #: 29649

Recommended E.coli strain for propagation: Stbl3

Selection: Ampicillin (100 ug/ml final concentration).

 

BirA in pET28a (w400-2)

details coming soon.


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