Future vectors

Vectors in this page were made, but they have not been fully characterized. These vectors might be available in the future, depending on the results obtained by various groups that are testing them.

Human telomerase reverse transcriptase (hTERT) in pENTR1A (w664-1)

High resolution pdf: hTERT in pENTR1A (w664-1).pdf

Sequence file: hTERT in pENTR1A (w664-1).seq

Sequence verification files: w664-1ENTRforw.ab1, w664-1ENTRrev.ab1

Addgene #: not released yet.

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin (50 ug/ml final concentration).

Compatible Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO

 

pLenti CMV hTERT Neo (w665-1)

High-resolution pdf: pLenti CMV hTERT Neo (w665-1).pdf

Sequence file: pLenti CMV hTERT Neo (w665-1).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin (100 ug/ml final concentration).

pLenti PGK hTERT Blast (w666-1)

High-resolution pdf: pLenti PGK hTERT Blast (w666-1).pdf

Sequence file: pLenti PGK hTERT Blast (w666-1).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin (100 ug/ml final concentration).

 

pENTR/pTER shDNA-PKcs #1 (726-1)

High-resolution pdf: pENTR/pTER shDNA-PKcs #1 (726-1).pdf

Sequence file: pENTR/pTER shDNA-PKcs #1 (726-1).seq

Sequence verification files: 726-1ENTRforw.ab1, 726-1ENTRrev.ab1

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pENTR/pTER shDNA-PKcs #2 (729-1)

High-resolution pdf: pENTR/pTER shDNA-PKcs #2 (729-1).pdf

Sequence file:

Sequence verification files:

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pLenti X1 Zeo/pTER shDNA-PKcs #1 (731-1)

High-resolution pdf: pLenti X1 Zeo/pTER shDNA-PKcs #1 (731-1).pdf

Sequence file:

Addgene #: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Zeocin (50 ug/ml) in low salt LB.

 

pLenti X1 Zeo/pTER shDNA-PKcs #2 (734-1)

High-resolution pdf:

Sequence file:

Recommended E.coli strain for propagation: Stbl3.

Selection: Zeocin (50 ug/ml) in low salt LB.

 

pENTR/pTER shMenin #1 (w173-1)

High-resolution pdf: pENTR/pTER shMenin #1 (w173-1).pdf

Sequence file:

Sequence verification file: w173-1ENTRrev.ab1

Addgene#: not released yet.

The shRNA sequence was obtained from Lin and Elledge (2003) Cell 113:881-9.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pLenti X2 Neo/pTER shMenin #1 (w180-1)

High-resolution pdf:

Sequence file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin (100 ug/ml final concentration).

 

pENTR/pSUPER shMenin #1 (299-2)

Sequence verification file: w299-2ENTRforw.ab1

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pLenti X2 Neo/pSUPER shMenin #1 (w312-1)

High-resolution pdf:

Sequence file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin (100 ug/ml final concentration).

 

pENTR/pTER shDP-1 #1 (w178-1)

High-resolution pdf: pENTR/pTER shDP-1 no 1.pdf

Sequence file:

Sequence verification files: w178-1ENTRrev.ab1, w178-1ENTRrev2.ab1

The shRNA sequence was obtained from Maehara et al. (2005) JCB 168:553-60.

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pLenti X2 Neo/pTER shDP-1 #1 (w183-1)

High-resolution pdf:

Sequence file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin (100 ug/ml final concentration).

 

pENTR/pTER shProhibitin #1 (w179-2)

High resolution pdf: pENTR/pTER shPHB1 no1 (w179-2).pdf

Sequence verification file: w179-2ENTRrev.ab1

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pLenti X2 Neo/pTER shProhibitin #1 (w182-1)

High-resolution pdf:

Sequence file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin (100 ug/ml final concentration).

 

Prohibitin (PHB1) in pENTR4-V5 (w202-1)

High-resolution pdf:

Sequence file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin (50 ug/ml final concentration).

 

pENTR/pTER shPIASgamma (PIAS4) #1 (w200-3)

High-resolution pdf: pENTR/pTER shPIAS4 #1 (w200-3).pdf

Sequence verification file: w200-3ENTRforw.ab1

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pLenti X2 Neo/pTER shPIASgamma (PIAS4) #1 (w205-1)

High-resolution pdf:

Sequence file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin (100 ug/ml final concentration).

 

PIASgamma (PIAS4) in pENTR-V5 (w212-1)

High-resolution pdf:

Sequence file:

Sequence verification files: w212-1ENTRforw.ab1, w212-1ENTRrev.ab1

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pLenti CMV/TO V5-PIASgamma (PIAS4) Hygro (w228-1)

High-resolution pdf:

Sequence file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin (100 ug/ml final concentration).

 

pENTR/pSM2 (CMV/TO) miHMGA1 #1(w313-2)

High-resolution pdf:

Sequence file:

Sequence verification file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pENTR/pSM2 (CMV/TO) miHMGA2 #1 (w314-1)

High-resolution pdf:

Sequence file:

Sequence verification file:

Addgene#: not released yet.

Recommended E.coli strain for propagation: TOP10F'

Selection: Kanamycin (50 ug/ml final concentration).

 

pLenti CMV GFP X2 DEST (w692-1)

High-resolution pdf: pLenti CMV GFP X2 DEST (w692-1).pdf

Sequence file: pLenti CMV GFP X2 DEST (w692-1).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: DB3.1 or ccDB survival.

Selection: Ampicillin (100 ug/ml final concentration) and Chloramphinecol (34 ug/ml).

 

pLenti CMV GFP PGK GFP X2 DEST (w691-1)

High-resolution pdf: pLenti CMV GFP PGK GFP X2 DEST (w691-1).pdf

Sequence file: pLenti CMV GFP PGK GFP X2 DEST (w691-1).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: DB3.1 or ccDB survival.

Selection: Ampicillin (100 ug/ml final concentration) and Chloramphinecol (34 ug/ml).

 

pLenti CMV PGK GFP DEST (w683-1)

High-resolution pdf: pLenti CMV PGK GFP DEST (w683-1).pdf

Sequence file: pLenti CMV PGK GFP DEST (w683-1).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: DB3.1 or ccDB survival.

Selection: Ampicillin (100 ug/ml final concentration) and Chloramphinecol (34 ug/ml).

 

pLenti CMV/TO PGK GFP DEST (w684-1)

High-resolution pdf: pLenti CMV/TO PGK GFP DEST (w684-1).pdf

Sequence file: pLenti CMV/TO PGK GFP DEST (w684-1).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: DB3.1 or ccDB survival.

Selection: Ampicillin (100 ug/ml final concentration) and Chloramphinecol (34 ug/ml).

 

pLenti X1 PGK GFP DEST (w685-2)

High-resolution pdf: pLenti X1 PGK GFP DEST (w685-2).pdf

Sequence file: pLenti X1 PGK GFP DEST (w685-2).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: DB3.1 or ccDB survival.

Selection: Ampicillin (100 ug/ml final concentration) and Chloramphinecol (34 ug/ml).

 

pLenti X1 GFP-Zeo DEST #2 (681-19)

High-resolution pdf: pLenti X1 GFP-Zeo DEST no2 (681-19).pdf

Sequence file:pLenti X1 GFP-Zeo DEST no 2 (681-19).seq

Addgene #: not released yet.

Recommended E.coli strain for propagation: DB3.1 or ccDB survival.

Selection: Zeocin (50 ug/ml final concentration) and Chloramphinecol (34 ug/ml) in low salt LB.

 

pENTR/U6tight XH (w761-1)

High resolution pdf: pENTR/U6tight XH (w761-1).pdf

Sequence file: pENTR/U6tight XH (w761-1).seq

Sequence verification files: w761-1ENTRforw.ab1

Addgene #: not released yet.

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin (50 ug/ml final concentration).

Compatible Destination vectors: pLenti X1, pLenti X2, pQCXI X2 series, pLenti CMV GFP.

 

pENTR/U6tight XH shLUC #2 (w757-1)

High resolution pdf: pENTR/U6tight XH shLUC #2 (w757-1).pdf

Sequence file: pENTR/U6tight XH shLUC #2 (w757-1).seq

Sequence verification files: w757-1ENTRforw.ab1

Addgene #: not released yet.

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin (50 ug/ml final concentration).

Compatible Destination vectors: pLenti X1, pLenti X2, pQCXI X2 series, pLenti CMV GFP.


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