News

October 9, 2016. Moving the web site to a new hosting site (again!). Some of the links might be broken, I will fix them as I go through the files, though it might take some time to get all the links working again.

June 2016. The pLenti CMVTRE3G GFP Series is again available at Addgene. However, the empty backbones (Destination vectors) are still not available. Check the GFP expression control page for more details

June 20, 2015. Moving web site to new hosting site. Hopefully the move will go smoothly, problems on June 20-21 should be temporary, let me know if there are still problems after Jun 21 (ecampeau@gmail.com).

June 20, 2015. MuLE Vectors for Customized Lentivirus Production, developed by the lab of Ian Frew, also available through Addgene (link here). Several of these vectors should be compatible with several vectors from this site, just make sure you know your Gateway cloning sites (overview link here).

February 6, 2012. The sequence file for the pENTR4-FLAG (w210-2) plasmid had a mistake. It is now corrected. The file on Addgene's web site did not contain the mistake.

I am slowly finishing the updates for the expression manual to contain all the vectors I released on Addgene's web site. No predicted release date yet.

September 6, 2011. The pLenti CMV rtTA3G Blast (R980-M38-658) vector is now available through Addgene (plasmid #31797). This plasmid can be used to make Tet-On3G cell lines for use with the CMVTRE3G series of vectors.

July 19, 2011. Added the vectors recently released to the lentiviral and retroviral schemes.

July 6, 2011. The pLenti CMVTRE3G Blast DEST (w803-2), pLenti CMVTRE3G Neo DEST (w813-1) and pENTR4-CLIPf (w877-2) have been released by Addgene today.

June 6, 2011. The pLenti CMVTRE3G GFP Blast (w818-1), pLenti CMVTRE3G GFP Neo (w821-1), pLenti CMVTRE3G GFP Puro (w819-1), pLenti CMVTRE3G Puro DEST (w811-1) and pLenti CMV rtTA3 Hygro (w785-1) have been released by Addgene today. The remaining plasmids should be available shortly.

May 11, 2011. The pENTR4-SNAPf (w878-1), pENTR4-HaloTag (w876-1), BirA in pENTR1A (w860-1) and pLenti PGK BirA Hygro (w874-1) are now available through Addgene.

April 27, 2011. The pENTR4-SB1 (w789-3) is now available through Addgene.

April 20, 2011. The maps and sequences for the CLIPf/SNAPf, HaloTag technologies as well as the SB1 epitope and the CMVTRE3G Series have been uploaded. These vectors should be available in late May/early June from Addgene.

April 17, 2011. I am not planing to make new vectors as I am no longer working in academia. However, I will maintain this web site and update it with new information about compatible vectors made by other groups, as long as the vectors are available through a public repository such as Addgene, PlasmID, or DNASU. Please find my new contact information here.

April 12, 2011. The last vectors made were sent to Addgene and should be available in 1-2 months. These include some Tet-On 3G vectors as well as some Entry vectors with the SB1 epitope, SNAP, CLIP and HaloTag fusions. However, for SNAPf, CLIPf, and HaloTag, no functional characterizations of the plasmids were made, though they were confirmed by sequencing. I will update the manuals as well as the map and sequence of each plasmid.

January 6, 2011. The other Tet-On Advanced vectors (including their GFP controls) are now available through Addgene. cDNAs cloned into Entry vectors can now be recombined with either T-REx or Tet-On Advanced Destination vectors. As mentioned in December, the latest generation of tetracycline-inducible vectors (Tet-On 3G) are currently being tested. If the tests are conclusive, they will be sent to Addgene later this month. The manual for the expression studies should be updated in the following weeks to include the Tet-On Advanced (and possibly the Tet-On 3G) vectors. A new interactive map is now active on the vector maps page. The old map will still be accessible.

December 17, 2010. The pLenti CMVtight Puro DEST (w768-1) (Addgene #26430)vector is now available at Addgene. This vector allows inducible expression of the protein of interest in Tet-On Advanced cells. The Tet-On Advanced system (Clontech) is the second generation of tetracycline-inducible system. The third generation (Tet-On 3G, link here) was released in July 2010. Lentiviral Destination vectors with the Tet-On 3G (CMVTRE3G) are made, they will be tested shortly.

November 13, 2010. The Entry vector pENTR4-SB1 (w789-3) is ready for testing. The SB1 epitope was characterized in Schaeffer et al. 2010 (NAR Apr;38(6):e91) as part of the SnAvi tag. The SB1 hybridoma cell line can be ordered from the Developmental Studies Hybridoma bank (link here). The tag remains to be tested in our hands.

November 4, 2010. The pQCXIR-DEST (Addgene #25433) made by Octavian Henegariu (Yale University) is now available through Addgene.

November 2, 2010. Minor updates to the manuals for expression and RNAi studies.

October 25, 2010. The pENTR4-GLU-GLU (w719-1) and pLenti CMV/TO GFP-MDC1 (779-2) plasmids are now available from Addgene.

October 18, 2010. The MDC1 in pENTR4 (330-5), pENTR-GFP-MDC1 (770-7) and pQCXIR (w498-1) plasmids are now available from Addgene and their links to Addgene's website have been updated.

September 28, 2010. A new interactive lentiviral vector map is available for trial. With the number of Destination vectors constantly increasing, it becomes hard to fit all the vectors into a workable scheme. Hopefully it will simplify the map and make space for future vectors. Feedbacks/suggestions are welcome.

September 26, 2010. The Tet-On Advanced vectors for expression are working. They will be sent to Addgene this week and should be available in October or November. The vectors can be found on the Tet-On Advanced vector page for the Destination vectors, and on the control vector page for the GFP controls. The menu for the vectors will be updated shortly.

September 9, 2010.The following vectors should be available soon from Addgene: pENTR4-GLU-GLU (w719-1), pENTR-GFP-MDC1 (770-7), pLenti CMV/TO GFP-MDC1 (779-2), MDC1 in pENTR4 (330-5), pLenti CMV GFP MCS (721-1), pQCXIG (w497-1), pQCXIR (w498-1). The pQCXIG DEST vector is now available.

September 1, 2010. Two new retroviral Destination vectors made by Octavian Henegariu (Yale University) should be available soon through Addgene: pQCXIG DEST and pQCXIR DEST.

August 24, 2010. UMass e-mail server is down. Please e-mail me at ecampeau@hotmail.com to contact me.

August 10, 2010. Tet-On advanced vectors for expression are made, we are currently testing them.

June 24, 2010. Some Tet-On advanced vectors for RNAi were added to the future vector section. Tet-On advanced vectors for expression should be available soon. A presentation about the lentiviral vector system will be given on July 16 at Nagoya University in Japan.

June 3, 2010. Vectors compatible with Clontech's Tet-On advanced system are currently being made. For expression, they will have the CMVtight promoter and for RNAi, the U6tight promoter. The pLenti CMV rtTA3 Blast (w756-1) vector that contains the reverse-transactivator 3 element is ready to be tested and is in the future vector section.

March 29, 2010. The pENTR4-GLU-GLU (w719-1) vector is ready to be tested in the future vector section. The GLU-GLU tag is an alternative to other tags and has very low background in the mammalian cell lines we tested by Western blot and immunofluorescence.

March 24, 2010. The University of Massachusette Medical School seems to have problems with their e-mail server. Some e-mails could not be sent and others were directly sent to the junk mail folder. If you don't hear from me after a few days, please e-mail me at ecampeau@hotmail.com to make sure I actually received the e-mail.

March 23, 2010. The manual with the protocols for the expression studies is finally out (version 1.0). Also, the option to join a mailing list was added and two presentations about this system will be given this Spring. Also added MIME type setting for Vector NTI files (.gb).

January 7, 2010. I will start including some vectors from other laboratories that complement well this system. The source of the vector will be acknowledged and these vectors are also available through Addgene. Please contact me if you find some vectors that could be added.

January 5, 2010. Happy new year to all ! Some of the PGK GFP Destination vectors are ready to be tested in the future vector section. The drug selection cassette was replaced with a PGK GFP cassette in the pLenti CMV, pLenti CMV/TO and pLenti X1 series to allow cell sorting and in vivo studies. The intensity of the GFP fluorescence should be much higher than in the GFP-Zeo fusion protein. Also, most if not all of the latest vectors are now available through Addgene. The sequence and map of the pQCXP CMV/TO LUC (w432-1), pQCXP CMV/TO V5-LUC (w476-1) and pQCXP CMV/TO empty (w368-1) was corrected (see October 6 note for more details).

December 17, 2009. Some of the cell cycle checkpoint vectors are now available through Addgene. The links have been posted on all the vectors and they should all be available in the next 1-2 weeks.

November 25, 2009. Fixed MIME problem for .ab1 and .seq file. Also, Addgene received the vectors against various cell cycle checkpoint proteins. The vectors should be available in 4-5 weeks. I will upload the information on these vectors as soon as the web pages are done.

November 24, 2009. Links to .ab1 and .seq not working. These extensions are not supported by the new host. I need to learn how to edit the web.config file to change MIME type settings.

November 23, 2009. Future vector section. Some future vectors are shown in this section. Feel free to contact me if you are interested in trying some of these vectors. Vectors for some cell cycle checkpoint proteins (p16, Rb, p53, MDC1) and other vectors will be sent this week to Addgene. They should become available in 2-3 weeks. The web site will be updated shortly.

October 6, 2009. New hosting server. The site was down due to my web hosting provider. I changed provider hoping it will solve the problem.

Correction to the sequence of plasmid w360-1 (pQCXP CMV/TO DEST). An insertion of 77 bp was removed after the attB2 site removing a Not I and Xho I site. It does not change anything for the LR recombination, only if the vector is to be used for traditional cloning. The map and sequence of the plasmid was updated.

September 17-19, 2009. The manual with the protocols for RNAi studies is now available.

Correction to the sequence of plasmids 696-6, 708-1, 709-1, 710-1 (pENTR/EF series). The sequence of the pENTR/EF vectors was changed due to a minor mistake during the assembly of the sequence file (thanks to Stefania Nicoli). It removes a Hinc II site before the EF-1 alpha promoter, but does not change anything else. The maps and sequences were updated. Please note that the maps and sequences on the Addgene web site are not updated yet. Also, I included the original sequence files (i.e. chromatograms) for the Entry vectors that were sequenced so everyone can see exactly was has been confirmed. Most of the Destination vectors were confirmed by restriction digest and shown to be functional in the article.

August 12, 2009. The vectors are now available through Addgene.

August 7, 2009. All the vector's maps and sequences are now available. The plasmids are not available from Addgene yet.

August 6, 2009. Official opening of the web site with the publication of the article. The web site should get better as I learn how to make web pages.

There are a few of the links that are not fully functional. I am still working on making a decent manual with detailed information and protocols. I will upload the missing information as soon as it will be finished. I will update this page as new information and links will be activated.


www.000webhost.com