Protein phosphatase 2A (PP2A) inhibition vectors

SV40 Small and Large T Antigen in pENTR1A (w611-7)

High-resolution pdf: SV40 small and large T antigen in pENTR1A (w611-7).pdf

Sequence file: SV40 small and large T antigen in pENTR1A (w611-7).seq

Sequence verification files:w611-7ENTRforw.ab1, w611-7ENTRrev.ab1

Addgene #:22297

Recommended E.coli strain for propagation: TOP10F'.

Selection: Kanamycin.

Compatible Destination vectors: pLenti CMV, CMV/TO, PGK series, pQCXI CMV/TO series, pQCXP CMV/TO

 

pLenti CMV/TO SV40 Small and Large T antigen (w612-1)

High-resolution pdf: pLenti CMV/TO SV40 small and large T antigen (w612-1).pdf

Sequence file: pLenti CMV/TO SV40 small and large T antigen (w612-1).seq

Addgene #:22298

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin.

 

Expression of the SV40 small and Large T antigen in MCF10A cells:

Lane 1: MCF10A transduced with a lentivirus derived from plasmid w612-1 at passage 0.

Lane 2: MCF10A transduced with a lentivirus derived from plasmid w612-1 at passage 4.

Lane 3: MCF10A untransduced (negative control).

Lane 4: VA13 (these cells are transformed with SV40, positive control).

Alpha-tubulin is used as a loading control.

The blot was probed with antibodies against alpha-tubulin (clone DM1a) and the SV40 Large T antigen (Ab-2, Thermo Scientific, clone PAb108, Cat. #MS-1833).


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