Tet-On Advanced Vectors

pLenti CMV rtTA3 Blast (w756-1)

High resolution pdf: pLenti CMV rtTA3 Blast (w756-1).pdf

Sequence file: pLenti CMV rtTA3 Blast (w756-1).seq

Addgene #: 26429

Recommended E.coli strain for propagation: Stbl3.

Selection: Ampicillin.

This vector can be used to make cell lines Tet-On Advanced. For more information about the Tet-On Advanced system, please visit Clontech's website (here). The rtTA3 element was a gift from Dr. Dominic Esposito.

 

pLenti CMVtight BLAST DEST (w762-1)

High resolution pdf:pLenti CMVtight Blast DEST (w762-1).pdf

Sequence file: pLenti CMVtight Blast DEST (w762-1).seq

Addgene #: 26434

Recommended E.coli strain for propagation before the LR reaction: DB3.1 or ccDB survival.

Selection before the LR reaction: Ampicillin AND Chloramphinecol.

Recommended E.coli strain for propagation after the LR reaction: Stbl3.

Selection after the LR reaction: Ampicillin.

Compatible Entry vectors: pENTR noccDB, pENTR with tags, degrons, NLS.

The CMVtight element was a gift from Dr. Dominic Esposito.

NOTE: If the vector pLenti CMV rtTA3 Blast (w756-1) was used to make the Tet-On cell line, this vector can not be selected for because the cell line will be already resistant to blasticidin. The rtTA3 element should be expressed under a different drug selection such as hygromycin using the pLenti CMV rtTA3 Hygro (w785-1) vector.

 

pLenti CMVtight Hygro DEST (w769-1)

High resolution pdf: pLenti CMVtight Hygro DEST (w769-1).pdf

Sequence file: pLenti CMVtight Hygro DEST (w769-1).seq

Addgene #: 26433

Recommended E.coli strain for propagation before the LR reaction: DB3.1 or ccDB survival.

Selection before the LR reaction: Ampicillin AND Chloramphinecol.

Recommended E.coli strain for propagation after the LR reaction: Stbl3.

Selection after the LR reaction: Ampicillin.

Compatible Entry vectors: pENTR noccDB, pENTR with tags, degrons, NLS.

The CMVtight element was a gift from Dr. Dominic Esposito.

NOTE: Do not use this vector if the Tet-On Advanced cell line was made with the pLenti CMV rtTA3 Hygro (w785-1) vector because the cell line will already be resistant to hygromycin.

 

pLenti CMVtight Neo DEST (w770-1)

High resolution pdf: pLenti CMVtight Neo DEST (w770-1).pdf

Sequence file: pLenti CMVtight Neo DEST (w770-1).seq

Addgene #: 26432

Recommended E.coli strain for propagation before the LR reaction: DB3.1 or ccDB survival.

Selection before the LR reaction: Ampicillin AND Chloramphinecol.

Recommended E.coli strain for propagation after the LR reaction: Stbl3.

Selection after the LR reaction: Ampicillin.

Compatible Entry vectors: pENTR noccDB, pENTR with tags, degrons, NLS.

The CMVtight element was a gift from Dr. Dominic Esposito.

 

pLenti CMVtight Puro DEST (w768-1)

High resolution pdf: pLenti CMVtight Puro DEST (w768-1).pdf

Sequence file: pLenti CMVtight Puro DEST (w768-1).seq

Addgene #: 26430

Recommended E.coli strain for propagation before the LR reaction: DB3.1 or ccDB survival.

Selection before the LR reaction: Ampicillin AND Chloramphinecol.

Recommended E.coli strain for propagation after the LR reaction: Stbl3.

Selection after the LR reaction: Ampicillin.

Compatible Entry vectors: pENTR noccDB, pENTR with tags, degrons, NLS.

The CMVtight element was a gift from Dr. Dominic Esposito.


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